||RegRNA in 5'-UTR
||Granulocyte colony-stimulating factor stem-loop destabilizing element (G-CSF_SLDE)
||Granulocyte colony-stimulating factor (G-CSF) is secreted in response to the inflammatory mediators IL-1 and TNF-alpha and by activated macrophages. The synthesis of G-CSF is regulated at the post-transcriptional level through the control of mRNA stability. In unstimulated cells G-CSF mRNA is unstable but becomes stabilized in response to IL-1 or TNF-alpha, and also in the case of monocytes and macrophages, in response to LPS. G-CSF mRNA contains two types of cis-acting destabilizing elements. In addition to several copies of the AU-rich element (ARE) it contains another destabilizing element whose activity resides in a stem-loop structure, whose consensus sequence has been determined by site-directed mutagenesis. The stem is 8 nt long (or 7 nt in chicken) with a conserved YAU loop. The destabilizing activity consists in the enhancing of deadenylation rate.
RNA destabilization by the granulocyte colony-stimulating factor stem-loop destabilizing element involves a single stem-loop that promotes deadenylation.
Mol Cell Biol 2002; , 1664-73   PubMed