RegRNA
A Regulatory RNA Motifs and Elements Finder
  Release 1.0, Jan 2006
 
 
 

Accession R0034
Feature Type RegRNA in 5'-UTR
Name Gamma interferon activated inhibitor of Ceruloplasmin mRNA translation (GAIT element)
Regulatory Motif
descr

	h5(minlen=5, maxlen=6, pair={"A:U","U:A","G:C","C:G","G:U","U:G"})

		ss(minlen=3, maxlen=4)

		h5(seq="^N$", pair={"A:U","U:A","G:C","C:G","G:U","U:G"})

			h5(seq="^D$", pair={"A:U","U:A","G:U","U:G"})

				h5(seq="^N$", pair={"A:U","U:A","G:C","C:G","G:U","U:G"})

					ss(minlen=5, maxlen=5)

				h3

			h3

		h3

		ss(minlen=0, maxlen=2)

	h3

Description Delayed translational silencing of ceruloplasmin (Cp) mRNA by gamma-interferon (IFN-gamma) is directed by a structural element in its 3' untranslated region. Translational silencing requires the binding of a cytosolic inhibitor to the Cp 3'UTR and all essential elements of mRNA circularization, i.e. eukaryotic initiation factor 4G, poly-A binding protein and poly-A tail. The 29-nt structural element, denoted as IFN-gamma-activated inhibitor of translation (GAIT), consisting of a 5-nt terminal loop, a weak 3-bp helix, an asymmetric internal bulge and a proximal 6-bp helix, has been experimentally demonstrated to be sufficient for translational silencing both in vitro and in vivo in human. A similar element can be found in sheep but not in rodents.
References
Transcript-selective translational silencing by gamma interferon is directed by a novel structural element in the ceruloplasmin mRNA 3 untranslated region.
Mol Cell Biol 2003; , 1509-19   PubMed 


Department of Biological Science and Technology, Institute of Bioinformatics, National Chiao Tung University, Taiwan
Contact with Dr. Hsien-Da Huang