A Regulatory RNA Motifs and Elements Finder
  Release 1.0, Jan 2006

Accession R0011
Feature Type RegRNA in 5'-UTR
Name Terminal Oligopyrimidine Tract (TOP)
Regulatory Motif
C yyyyyyyyyyyyyy[0,11,0]G
Description All vertebrate ribosomal protein and translation elongation factors analyzed to date contain a 5' terminal oligopyrimidine tract consisting of 5-15 pyrimidines (Kato et al., 1994) that is required for coordinate translational repression (Levy et al., 1991; Kaspar et al., 1992) during growth arrest, differentiation, development and certain drug treatments (reviewed in Kaspar et al., 1993; Meyuhas et al., 1996 ). This motif begins with a C and is followed by a G residue (consensus sequence C(Py)nG where n=3-14 pyrimidines); otherwise, no other requirements appear to be necessary except for consecutive pyrimidines. Deletion of the pyrimidine tract or exchanging purines for pyrimidines results in unregulated translation (Levy et al., 1991; Kaspar et al., 1992) . A protein of ~56 kDa was found which binds to the leaders of ribosomal proteins L32 and L1 but not to similar mutant RNAs lacking an intact polypyrimidine tract required for translational repression (Kaspar et al., 1992; Cardinali et al., 1993) . Recent results in a Xenopus system suggest that the La autoantigen may bind to the polypyrimidine motif and be involved in translational regulation (Pellizzoni et al., 1996).
References Kaspar RL, Kakegawa T, Cranston H, Morris DR, and White MW
A regulatory cis element and a specific binding factor involved in the mitogenic control of murine ribosomal protein L32 translation.
J Biol Chem 1992; 267(1), 508-14   PubMed 

Levy S, Avni D, Hariharan N, Perry RP, and Meyuhas O
Oligopyrimidine tract at the 5 end of mammalian ribosomal protein mRNAs is required for their translational control.
Proc Natl Acad Sci U S A 1991; 88(8), 3319-23   PubMed 

Kato S, Sekine S, Oh SW, Kim NS, Umezawa Y, Abe N, Yokoyama-Kobayashi M, and Aoki T
Construction of a human full-length cDNA bank.
Gene 1994; 150(2), 243-50   PubMed 

Cardinali B, Di Cristina M, and Pierandrei-Amaldi P
Interaction of proteins with the mRNA for ribosomal protein L1 in Xenopus: structural characterization of in vivo complexes and identification of proteins that bind in vitro to its 5 UTR.
Nucleic Acids Res 1993; 21(10), 2301-8   PubMed 

Pellizzoni L, Cardinali B, Lin-Marq N, Mercanti D, and Pierandrei-Amaldi P
A Xenopus laevis homologue of the La autoantigen binds the pyrimidine tract of the 5 UTR of ribosomal protein mRNAs in vitro: implication of a protein factor in complex formation.
J Mol Biol 1996; 259(5), 904-15   PubMed 

Xu N, Chen CY, and Shyu AB
Modulation of the fate of cytoplasmic mRNA by AU-rich elements: key sequence features controlling mRNA deadenylation and decay.
Mol Cell Biol 1997; 17(8), 4611-21   PubMed 

Department of Biological Science and Technology, Institute of Bioinformatics, National Chiao Tung University, Taiwan
Contact with Dr. Hsien-Da Huang